Tropomyosin Ser-283 pseudo-phosphorylation slows myofibril relaxation

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Specific phosphorylation at serine-283 of alpha tropomyosin from frog skeletal and rabbit skeletal and cardiac muscle.

Tropomyosin, extracted from the leg muscle of frogs that had been injected with [32P]orthophosphate, was fractionated into two components, alpha and beta, on a CM-cellulose column. Radioactivity was associated only with the alpha component. A single phosphorylation site was located at serine-283 (pentultimate at the COOH-terminal end) of the frog alpha tropomyosin. The same phosphorylated pepti...

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ژورنال

عنوان ژورنال: Archives of Biochemistry and Biophysics

سال: 2013

ISSN: 0003-9861

DOI: 10.1016/j.abb.2012.11.010